Comparative Transcriptome Analysis Reveals the Molecular Mechanism of UV-B Irradiation in Promoting the Accumulation of Phenolic Compounds in Wounded Carrot

Ultraviolet B (UV-B) irradiation has been demonstrated to play a synergistic effect with wounding in enhancing the accumulation of phenolic antioxidants carrots. However, little is known about molecular mechanisms UV-B treatment on wounded In this study, carrots were exposed different doses light, then stored at 15 °C for 2 d. The results showed that content compounds 1 KJ m?2 UV-B–treated samples was 415% and 247% higher than whole carrots, respectively. Based this, selected as optimal dose used further analysis. addition, greatly enhanced enzyme activity phenylalanine ammonia lyase (PAL) contents profiles, especially 3-O-caffeoylquinic acid (3-CQA). Transcriptome analysis found could accelerate KEGG pathways involved signal transduction secondary substance metabolism. differentially expressed genes (DEGs) phenolics biosynthesis–related metabolic processes shikimate pathway phenylpropanoid biosynthesis extensively upregulated by treatment. Our provided fundamental information better understanding regulation mechanism promoting